Plant Transcription Factor Database
Previous version: v3.0
Dorcoceras hygrometricum
HSF Family
Species TF ID Description
KZV15896.1HSF family protein
KZV17100.1HSF family protein
KZV18523.1HSF family protein
KZV21607.1HSF family protein
KZV22125.1HSF family protein
KZV23490.1HSF family protein
KZV24953.1HSF family protein
KZV28447.1HSF family protein
KZV28523.1HSF family protein
KZV28960.1HSF family protein
KZV30235.1HSF family protein
KZV31065.1HSF family protein
KZV34147.1HSF family protein
KZV34879.1HSF family protein
KZV37269.1HSF family protein
KZV40964.1HSF family protein
KZV45161.1HSF family protein
KZV46906.1HSF family protein
KZV47502.1HSF family protein
KZV48527.1HSF family protein
KZV50563.1HSF family protein
KZV50691.1HSF family protein
KZV51075.1HSF family protein
KZV51966.1HSF family protein
KZV54169.1HSF family protein
KZV54573.1HSF family protein
KZV56297.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645